在厨房被c到高潮a毛片奶水_伊人天堂av无码av日韩av_午夜亚洲av永久无码精品_欧美xxxx色视频在线观看_国产韩国精品一区二区三区

咨詢熱線

15000266580

當(dāng)前位置:首頁(yè) >產(chǎn)品中心>細(xì)胞庫(kù)>動(dòng)物腎細(xì)胞>CRL-1650COS-1 非洲綠猴SV40轉(zhuǎn)化的腎細(xì)胞

COS-1 非洲綠猴SV40轉(zhuǎn)化的腎細(xì)胞

簡(jiǎn)要描述:CRL-1650 COS-1 非洲綠猴SV40轉(zhuǎn)化的腎細(xì)胞,ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|,細(xì)胞庫(kù)管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和*培養(yǎng)條件

  • 產(chǎn)品型號(hào):CRL-1650
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2024-11-14
  • 訪  問(wèn)  量:1918

產(chǎn)品分類(lèi)

Product Category

詳細(xì)介紹

CRL-1650  COS-1 非洲綠猴SV40轉(zhuǎn)化的腎細(xì)胞,ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|,細(xì)胞庫(kù)管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)優(yōu)培養(yǎng)條件


CRL-1650  COS-1 非洲綠猴SV40轉(zhuǎn)化的腎細(xì)胞 的詳細(xì)介紹


ATCC® Number:  CRL-1650™

Designations:  COS-1

Depositors:   Y Gluzman

Biosafety Level: 2 [Cells Contain PAPOVAVIRUS ]

Shipped:  frozen

Medium & Serum:  See Propagation

Growth Properties: adherent

Organism: Cercopithecus aethiops

Morphology: fibroblast


Source: Organ: kidney

Cell Type: SV40 transformed

Cellular Products: T antigen

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Applications: transfection host (Nucleofection technology from Lonza

Roche FuGENE® Transfection Reagents)

Virus Susceptibility: SV40 (lytic growth); SV40 tsA209 at 40C; SV40 mutants with deletions in the early region

Comments: This is an African green monkey kidney fibroblast-like cell line suitable for transfection by vectors requiring expression of SV40 T antigen. This line contains T antigen, retains complete permissiveness for lytic growth of SV40, supports the replication of ts A209 virus at 40C, and supports the replication of pure populations of SV40 mutants with deletions in the early region.The line was derived from the CV-1 cell line (ATCC ® CCL-70?) by transformation with an origin defective mutant of SV40 which codes for wild type T antigen. The cells contain a single integrated copy of the complete early region of the SV40 genome.

Propagation:  ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Subculturing:  Protocol:

Remove and discard medium.

Briefly rinse the cell layer with 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.

Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually 5 to 10 min).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detatch. Cells that are difficult to detatch may be placed at 37C to facilitate dispersal.

Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

Add appropriate aliquots of the cell suspension to new culture vessels.

Incubate cultures at 37C.

Subc*tion Ratio: A subc*tion ratio of 1:4 to 1:8 is recommended

Medium Renewal: 2 to 3 times per week


Preservation:  Freeze medium: Complete growth medium 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor temperature

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002

recommended serum:ATCC 30-2020

parental cell line:ATCC CCL-70

0.25% (w/v) Trypsin - 0.53 mM EDTA in Hank' BSS (w/o Ca++, Mg++):ATCC 30-2101

Cell culture tested DMSO:ATCC 4-X

References: 1822: Gluzman Y. SV40-transformed simian cells support the replication of early SV40 mutants. Cell 23: 175-182, 1981. PubMed: 6260373

32348: Mansky LM. The mutation rate of human immunodeficiency virus type 1 is influenced by the vpr gene. Virology 222: 391-400, 1996. PubMed: 8806523

32368: Churchill MJ, et al. The rev-responsive element negatively regulates human immunodeficiency virus type 1 env mRNA expression in primate cells. J. Virol. 70: 5786-5790, 1996. PubMed: 8709194

32373: Goodrum FD, et al. Adenovirus early region 4 34-kilodalton protein directs the nuclear localization of the early region 1B 55-kilodalton protein in primate cells. J. Virol. 70: 6323-6335, 1996. PubMed: 8709260

32555: Suss-Toby E, et al. Toxoplasma invasion: the parasitophorous vacuole is formed from host cell plasma membrane and pinches off via a fission pore. Proc. Natl. Acad. Sci. USA 93: 8413-8418, 1996. PubMed: 8710885

32582: Chang K, Pastan I. Molecular cloning of mesothelin, a differentiation antigen present on mesothelium, mesotheliomas, and ovarian cancers. Proc. Natl. Acad. Sci. USA 93: 136-140, 1996. PubMed: 8552591

32788: Lu FM, Lux SE. Constitutively active human notch 1 binds to the transcription factor CBF1 and stimulates transcription through a promoter containing a CBF1-responsive element. Proc. Natl. Acad. Sci. USA 93: 5663-5667, 1996. PubMed: 8643633

32972: Bhattacharyya DK, et al. Involvement of arginine 120, glutamate 524, and tyrosine 355 in the binding of arachidonate and 2-phenylpropionic acid inhibitors to the cyclooxygenase active site of ovine prostaglandin endoperoxide H synthase-1. J. Biol. Chem. 271: 2179-2184, 1996. PubMed: 8567676

33048: Feng XH, Derynck R. Ligand-independent activation of transforming growth factor (TGF) beta-signaling pathways by heteromeric cytoplasmic domains of TGF-beta receptors. J. Biol. Chem. 271: 13123-13129, 1996. PubMed: 8662796

33149: Wang LH, et al. Identification of thromboxane A2 synthase active site residues by molecular modeling-guided site-directed mutagenesis. J. Biol. Chem. 271: 19970-19975, 1996. PubMed: 8702713

33176: Almaula N, et al. Mapping the binding site pocket of the serotonin 5-hydroxytryptamine 2A receptor. J. Biol. Chem. 271: 14672-14675, 1996. PubMed: 8663249



CRL-1650  



















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說(shuō)明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫(xiě)阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
国产无遮挡又黄又爽在线视频| 国产精品一在线观看| 人妻丰满熟妇av无码区免| 毛片资源精品在线观看| 国产成人无码视频一区二区三区 | 久久精品夜色国产亚洲av| 夜夜夜高潮夜夜爽夜夜爰爰 | 久久精品无码一区二区三区| 亚洲av影院一区二区三区四区| 国产女人水真多18毛片18精品| 五月花成人网| 成人免费无码大片a毛片抽搐色欲| av在线网站无码不卡的| 免费无遮挡无码永久在线观看视频| 在线观看特色大片免费视频| 色偷偷av男人的天堂| 麻豆国产人妻欲求不满| 吃奶呻吟打开双腿做受动态图| 亚洲成av人片在线播放无码| 中文字幕无码一区二区免费| 国产亚洲av人片在线播放| 麻花传媒mv在线观看| 亚洲无码在线播放| 成人无码a级毛片免费| 亚洲av午夜精品无码专区| 亚洲天堂男人影院| 伊是香蕉大人免费一区| 成人性能视频在线| 熟妇人妻中文字幕无码老熟妇| 国产美女亚洲精品久久久综合| 天天燥日日燥| 国产精品综合av一区二区国产馆| 免费可以在线看a∨网站| 少妇交换黑人做爰| 欧美日韩亚洲中文字幕一区二区三区 | 性xxxx18免费观看视频| 人人妻人人澡人人爽欧美一区双 | 一出一进一爽一粗一大视频免费的 | 久久国产精品偷| 大肉大捧一进一出好爽| a级大胆欧美人体大胆666|