在厨房被c到高潮a毛片奶水_伊人天堂av无码av日韩av_午夜亚洲av永久无码精品_欧美xxxx色视频在线观看_国产韩国精品一区二区三区

咨詢熱線

15000266580

當(dāng)前位置:首頁 >產(chǎn)品中心>細(xì)胞庫>小鼠腫瘤細(xì)胞、癌細(xì)胞>CCL-1.3 L-M(TK-小鼠胸腺激酶缺陷細(xì)胞株

CCL-1.3 L-M(TK-小鼠胸腺激酶缺陷細(xì)胞株

簡要描述:CCL-1.3 L-M(TK-) 小鼠胸腺激酶缺陷細(xì)胞株,
CCL-1.3 L-M(TK-小鼠胸腺激酶缺陷細(xì)胞株
ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|,
細(xì)胞庫管理規(guī)范,
提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和*培養(yǎng)條件

  • 產(chǎn)品型號:
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2024-11-14
  • 訪  問  量:2350

產(chǎn)品分類

Product Category

詳細(xì)介紹

CCL-1.3 L-M(TK-) 小鼠胸腺激酶缺陷細(xì)胞株,ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|,細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和i優(yōu)培養(yǎng)條件


CCL-1.3 L-M(TK-) 小鼠胸腺激酶缺陷細(xì)胞株

ATCC® Number: CCL-1.3™    Price: $329.00
Designations: L-M(TK-) [LM(tk-), LMTK-]

Depositors: TR Chen

Biosafety Level:1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties:adherent

Organism:Mus musculus (mouse)

Morphology:fibroblast


Source:Tissue: subcutaneous connective tissue; areolar and adipose
Strain: C3H/An


Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
CCL-1.3 L-M(TK-) 小鼠胸腺激酶缺陷細(xì)胞株

Applications:transfection host (Roche FuGENE® Transfection Reagents)

Tumorigenic:Yes

Antigen Expression:H-2k

Cytogenetic Analysis:modal number = 45; range = 42 to 48.

The stemline chromosome number is hyperdiploid with the 2S component occurring at 3.8%. Twenty-one markers were common to nearly all S metaphases studied. Of these, 13 were two-armed chromosomes, and eight, acrocentric. Four markers were isochromosomes by Robertsonian translocations from chromosomes 4, 12, 14 and 15. Normal X, 12, 14, 15 and 16 were absent. The X chromosome was present as an arm of Rb(X.3). (See Cancer Genet. Cytogenet. 24: 95-107, 1987 for a detailed karyotype description.)



Age: 100 days

Gender: male

Comments:The cells are 5-bromo-2'-deoxyuridine resistant (BUDR, BrdUrd) and thymidine kinase (TK) deficient.

HAT sensitive.

Tested and found negative for ectromelia virus (mousepox).



Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%


Subculturing: Protocol:
  1. Remove and discard culture medium.

  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.


  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

  5. Add appropriate aliquots of the cell suspension to new culture vessels.

  6. Incubate cultures at 37°C.


Subc*tion Ratio: A subc*tion ratio of 1:4 to 1:8 is recommended
Medium Renewal: Twice per week


Preservation: Freeze medium: complete growth medium, 95%; DMSO, 5%

Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002

recommended serum:ATCC 30-2020

parental cell line:ATCC CCL-1



References:868: Kit S, et al. Deletion of thymidine kinase activity from L cells resistant to bromodeoxyuridine. Exp. Cell Res. 31: 297-312, 1963. PubMed: 14056989

21410: Chen TR. Cytogenetics of somatic cell hybrids. II. Modal karyotypes and mosaicism in mouse mutant cell lines: LM(tk-), A9, and Rag. Cancer Genet. Cytogenet. 24: 95-107, 1987. PubMed: 3466673

25904: Hsu TC, Somers CE. Properties of L cells resistant to 5-bromodeoxyuridine. Exp. Cell Res. 26: 404-410, 1962. PubMed: 14449548

25907: Boone C, et al. Assignment of three human genes to chromosomes (LDH-A to 11, TK to 17, and IDH to 20) and evidence for translocation between human and mouse chromosomes in somatic cell hybrids (thymidine kinase-lactate dehydrogenase A-isocitrate dehydrogenase-C-11, E-17, and F-20 chromosomes). Proc. Natl. Acad. Sci. USA 69: 510-514, 1972. PubMed: 4110482

25910: Peterson JL, McBride OW. Cotransfer of linked eukaryotic genes and efficient transfer of hypoxanthine phosphoribosyltransferase by DNA-mediated gene transfer. Proc. Natl. Acad. Sci. USA 77: 1583-1587, 1980. PubMed: 6929511




















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說明:

  • 驗(yàn)證碼:

    請輸入計(jì)算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號:滬ICP備10013034號-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
大学生粉嫩无套流白浆| 脱岳裙子从后面挺进去在线观看 | 熟妇人妻中文字幕| 7777精品伊人久久久大香线蕉| 国产精品偷窥熟女精品视频| 草草影院发布页| 少妇被又大又粗又爽毛片| 亚洲欧美国产毛片在线| 欧美成人午夜精品久久久| 狠狠的干性视频| 成人精品视频一区二区三区尤物| 久久天天躁狠狠躁夜夜爽| 蜜桃传媒mv在线免费| 国产99久久久国产精品免费看| 超碰成人av免费观看| 老子午夜精品无码| 亚洲爆乳精品无码AAA片| 国产精品9999久久久久| 亚洲AV永久无码精品蜜芽| 国产欧美日韩精品a在线观看| 成午夜精品一区二区三区| 国产丰满麻豆videossexhd| 精品少妇人妻久久免费| 中文人妻熟妇乱又伦精品| 日本一区二区三区在线看| 2018高清国产一区二区三区| 一区二区三区内射美女毛片| 亚洲色欲啪啪久久www综合网| 国产精品久久久久久妇女6080| 对白脏话肉麻粗话av| 少妇高潮喷水在线观看| 十八禁无遮挡99精品国产| 日本免费一本天堂在线| 欧美激情精品久久久久久 | 中国丰满熟妇av| 四虎影视在线永久免费观看| 成人高潮视频在线观看| 欧美国产日韩a在线视频| 在线看亚洲十八禁网站| 国产精品成人VA在线播放| 人妻少妇精品视频三区二区一区|