在厨房被c到高潮a毛片奶水_伊人天堂av无码av日韩av_午夜亚洲av永久无码精品_欧美xxxx色视频在线观看_国产韩国精品一区二区三区

咨詢熱線

15000266580

當(dāng)前位置:首頁 >產(chǎn)品中心>細(xì)胞庫>人腫瘤細(xì)胞、癌細(xì)胞>HTB-148H4細(xì)胞,人腦神經(jīng)膠質(zhì)瘤細(xì)胞.

H4細(xì)胞,人腦神經(jīng)膠質(zhì)瘤細(xì)胞.

簡要描述:H4細(xì)胞,人腦神經(jīng)膠質(zhì)瘤細(xì)胞.
原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種;細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和*培養(yǎng)條件!

  • 產(chǎn)品型號:HTB-148
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時間:2024-11-21
  • 訪  問  量:1812

產(chǎn)品分類

Product Category

詳細(xì)介紹

H4細(xì)胞,人腦神經(jīng)膠質(zhì)瘤細(xì)胞.

ATCC® Number:HTB-148™    Price:$338.00
Designations:H4

Depositors:J Riggs

Biosafety Level:1

Shipped:frozen

Medium & Serum:See Propagation

Growth Properties:adherent

Organism:Homo sapiens (human)

Morphology:epithelial


Source:Organ: brain
Disease: neuroglioma


Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
H4細(xì)胞,人腦神經(jīng)膠質(zhì)瘤細(xì)胞.

Applications:transfection host (Roche FuGENE® Transfection Reagents)

Tumorigenic:No

DNA Profile (STR):Amelogenin: X,Y

CSF1PO: 10,12

D13S317: 12

D16S539: 11,12

D5S818: 10,12

D7S820: 8,11

THO1: 7,9

TPOX: 8,11

vWA: 14,18



Cytogenetic Analysis:modal number = 73; range = 63 to 78.

This is a hypertriploid human cell line having the modal chromosome number of 73 occurring in 26% of cells. However, cells having 75 chromosomes also occurred at a high rate (24%). Higher ploidies were found at 0.4%. At least 16 marker chromosomes are common to all metaphases examined: paired del(2)(p2209) and del(9)(p22) and single der(14)t(1;14)(p22;q22), del(3)(p2501), del(7)(q3209), del(10)(p1301), t(13q17q), t(3p13q) and at least eight others. The del(5) (p13), del(7) (q11) and a few others occurred in some, and many others were seen only once. N7 and N21 occurred in 4 or more copies per cell. Most cells had two X and two Y chromosomes.



Isoenzymes:AK-1, 1

ES-D, 1

G6PD, B

GLO-I, 2

Me-2, 0

PGM1, 1-2

PGM3, 1



Age:37 years

Gender:male

Ethnicity:Caucasian

Propagation:ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C


Subculturing:Protocol:              
  1. Remove and discard culture medium.

  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

  5. Add appropriate aliquots of the cell suspension to new culture vessels.

  6. Incubate cultures at 37°C.


    1. Subc*tion Ratio: A subc*tion ratio of 1:10 to 1:15 is recommended
      Medium Renewal: 2 to 3 times per week



Preservation:Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase


Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002

recommended serum:ATCC 30-2020



References:22287: Arnstein P, et al. Propagation of human tumors in antithymocyte serum-treated mice. J. Natl. Cancer Inst. 52: 71-84, 1974. PubMed:4544026
22907: Day RS, Ziolkowski CH. Human brain tumour cell strains with deficient host-cell reactivation of N-methyl-N'-nitro-N-nitrosoguanidine-damaged adenovirus 5. Nature 279: 797-799, 1979. PubMed: 450131

















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補充說明:

  • 驗證碼:

    請輸入計算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號:滬ICP備10013034號-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
日本少妇毛茸茸高潮| 午夜福利三级理论电影| 亚洲自拍高清国产九色| 中文字幕人成人乱码亚洲电影| 亚洲午夜精品a片久久www慈禧| 成年美女黄网站色大片免费看| 国精产品一二三区精华液| 欧美freesex黑人又粗又大| 久久国产精品福利一区二区三区 | 欧美黑人粗暴多交高潮水最多| 国产最新进精品视频| 国精一二二产品无人区免费应用| 欧美成人性视频在线播放| 无码精品a∨在线观看中文| 国产精品爱久久久久久久电影蜜臀 | 亚洲av无码乱码国产精品| 性色av一二三天美传媒| 国产 亚洲 制服 无码 中文| 亚洲护士一区二区三区| 自慰系列无码专区| 日韩免费视频一一二区| 欧美人与物videos另类xxxxx | 亚洲国产三级在线观看| 亚洲熟妇久久精品| 色妞色综合久久夜夜| 亚洲熟妇无码av另类vr影视| 国产精品人人做人人爽| 中国孕妇变态孕交xxxx| 无码性午夜视频在线观看| 成人高潮视频在线观看| 激情内射人妻1区2区3区| 国产亚洲欧美日韩二三线| 久久亚洲av无码精品色午夜麻豆| 无码中文字幕va精品影院 | 久久久久人妻精品一区二区三区| 色综合久久88色综合天天| 国产欧美精品区一区二区三区| 最近中文字幕mv在线视频看| 影音先锋男人av橹橹色| 一级做a爱视频在线播放| 日本熟妇厨房bbw|