在厨房被c到高潮a毛片奶水_伊人天堂av无码av日韩av_午夜亚洲av永久无码精品_欧美xxxx色视频在线观看_国产韩国精品一区二区三区

咨詢熱線

15000266580

當(dāng)前位置:首頁(yè) >產(chǎn)品中心>細(xì)胞庫(kù)>人正常細(xì)胞>HUVECHUV-EC細(xì)胞, 人臍靜脈內(nèi)皮細(xì)胞

HUV-EC細(xì)胞, 人臍靜脈內(nèi)皮細(xì)胞

簡(jiǎn)要描述:HUV-EC-C細(xì)胞, 人臍靜脈內(nèi)皮細(xì)胞
ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|,細(xì)胞庫(kù)管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和*培養(yǎng)條件

  • 產(chǎn)品型號(hào):HUVEC
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2024-11-16
  • 訪  問(wèn)  量:2088

產(chǎn)品分類

Product Category

詳細(xì)介紹

HUV-EC-C細(xì)胞, 人臍靜脈內(nèi)皮細(xì)胞

ATCC® Number:CRL-1730™    Price:$280.00
DeBiosSesignations:HUV-EC-C

rum:afety Level:1

Shipped:frozen

Medium &See Propagation

Growth Properties:adherent

Organism:Homo sapiens (human)

Morphology:endothelial

Source:Organ: umbilical vein
Tissue: vascular endothelium
Disease: normal
Cell Type: endothelial


Cellular Products:factor VIII [23284]

Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
HUV-EC-C細(xì)胞, 人臍靜脈內(nèi)皮細(xì)胞

Applications:transfection host (technology from amaxa)

Tumorigenic:No

DNA Profile (STR):Amelogenin: X

CSF1PO: 11,12

D13S317: 9,11

D16S539: 11,12

D5S818: 11,12

D7S820: 8,12

THO1: 6,9.3

TPOX: 8,11

vWA: 16



Cytogenetic Analysis:This is a hypodiploid human cell line. The modal chromosome number was 45 occurring in 72% of cells counted. The rate of polyploid cells was 15.8%. All cells had monosomic N13 and the subclone with additional monosomic N15 predominates. Other coexisting subclones include those with 46,XX,-11,-13,i(11p),i(11q) and 46,XX,+11,-13 karyotypes. Both X chromosomes appear normal.

Comments:Endothelial Cell Growth Supplement (ECGS) and unidentified factors from bovine pituitary, hypothalamus or whole brain extracts are mitogenic for this line. [23284]

The cells have a life expectancy of 50 to 60 population doublings.



Propagation:ATCC complete growth medium: The base medium for this cell line is ATCC-formulated of F-12K Medium, Catalog No. 30-2004. To make the complete growth medium, add the following components to the base medium: 0.1 mg/ml heparin; 0.03-0.05 mg/ml endothelial cell growth supplement (ECGS); adjust to a final concentration of 10% fetal bovine serum.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C


Subculturing:Protocol:
  1. Remove and discard culture medium.

  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.

  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

  5. Add appropriate aliquots of the cell suspension to new culture vessels.

  6. Incubate cultures at 37°C.


Subc*tion Ratio: A subc*tion ratio of 1:2 to 1:3 is recommended
Medium Renewal: Two to three times per week


Preservation:Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase


Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2004

recommended serum:ATCC 30-2020



References:23284: Hoshi H, McKeehan WL. Brain- and liver cell-derived factors are required for growth of human endothelial cells in serum-free culture. Proc. Natl. Acad. Sci. USA 81: 6413-6417, 1984. PubMed: 6333682
29192: Zahedi K. Characterization of the binding of serum amyloid P to laminin. J. Biol. Chem. 272: 2143-2148, 1997. PubMed: 8999915
33021: Soker S, et al. Characterization of novel vascular endothelial growth factor (VEGF) receptors on tumor cells that bind VEGF165 via its exon 7-endoded domain. J. Biol. Chem. 271: 5761-5767, 1996. PubMed:8621443























產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說(shuō)明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
国产精品18久久久久久麻辣| 中文字幕va一区二区三区| 欧美性白人极品1819hd| 国产精品热久久高潮av袁孑怡| 被三个男人绑着躁我好爽视频| 欧美成人午夜免费影院手机在线看| 国产精品乱码人妻一区二区三区 | 69sex久久精品国产麻豆| 一本大道中文日本香蕉| 高清中文字幕在线a片| 欧美一区二区三区激情| 成人黄色精品一区二区| 亚洲国产精品13p| 亚洲 欧美 中文 在线 视频 | 2021亚洲国产精品无码| 亚洲av午夜国产精品无码中文字| 在线观看视频午夜国产| 北条麻妃国产九九九精品视频| 极品人妻被黑人中出种子| 中文字人妻熟女理论片| 玩弄丰满少妇视频| 少妇av一区二区三区无码| 国产黄大片在线观看画质优化| 精品人妻无码一区二区色欲产成人| 久久这里都是精品一区| 欧美老妇与zozoz0交| 收集最新中文国产中文字幕| 四虎国产精品成人免费久久| 男同gay毛片免费可播放| 久久综合88熟女人妻| 沈阳熟女露脸对白视频 | 一级少妇人妻免费视频| 无码中文人妻在线一区二区三区 | 国产太嫩了在线观看| 十八18禁国产精品www| 性色av蜜臀av色欲av| 天天干天天射天天操| 色噜噜噜亚洲男人的天堂| 亚洲一区二区三区四区五区六 | 成人免费xxxxx在线观看| 天堂√最新版中文在线地址|