在厨房被c到高潮a毛片奶水_伊人天堂av无码av日韩av_午夜亚洲av永久无码精品_欧美xxxx色视频在线观看_国产韩国精品一区二区三区

咨詢熱線

15000266580

當(dāng)前位置:首頁 >產(chǎn)品中心>細(xì)胞庫>人正常細(xì)胞>CRL-2190HK-2 細(xì)胞,人腎近曲小管上皮細(xì)胞

HK-2 細(xì)胞,人腎近曲小管上皮細(xì)胞

簡要描述:HK-2 細(xì)胞,人腎近曲小管上皮細(xì)胞
原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種;細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和*培養(yǎng)條件!

  • 產(chǎn)品型號:CRL-2190
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時間:2024-11-15
  • 訪  問  量:2025

產(chǎn)品分類

Product Category

詳細(xì)介紹

HK-2 細(xì)胞,人腎近曲小管上皮細(xì)胞

ATCC® Number:CRL-2190™    Price:$272.00
Designations:HK-2

Depositors:RA Zager

Biosafety Level:2 [Cells Contain Papilloma viral DNA sequences ]

Shipped:frozen

Medium & Serum:See Propagation

Growth Properties:adherent

Organism:Homo sapiens (human)

Morphology:epithelial


Source:Organ: kidney, cortex
Tissue: proximal tubule
Cell Type: human papillomavirus 16 (HPV-16) transformed


Cellular Products:alkaline phosphatase; gamma glutamyltranspeptidase; leucine aminopeptidase; acid phosphatase; cytokeratin; alpha 3, beta 1 integrin; fibronectin

Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
HK-2 細(xì)胞,人腎近曲小管上皮細(xì)胞

Receptors:epidermal growth factor (EGF), expressed

DNA Profile (STR):Amelogenin: X,Y

CSF1PO: 13

D13S317: 9

D16S539: 11,12

D5S818: 12

D7S820: 10,11

THO1: 9

TPOX: 8,9

vWA: 17,18



Age:*****

Gender:male

Comments:HK-2 (human kidney 2) is a proximal tubular cell (PTC) line derived from normal kidney.

The cells were immortalized by transduction with human papilloma virus 16 (HPV-16) E6/E7 genes.

The recombinant retrovirus vector pLXSN 16 E6/E7 containing the HPV-16 E6/E7 genes was used to transfect the ectotropic packaging cell line Psi-2.

Virus produced by the Psi-2 cells was used to infect the amphotropic packaging cell line PA317 (see ATCC CRL-9078).

Virus produced by the PA317 cells was used to transduce primary PTCs.

Although pLXSN 16 E6/E7 also confers resistance to neomycin, selection in G418 was not used to isolate transduced clones.

The cell line appears to be derived from a single cell based on Southern and FISH analysis.

The E6/E7 genes are present in the HK-2 genome as determined by PCR.

The cells retain a phenotype indicative of well differentiated PTCs.

They are positive for alkaline phosphatase, gamma glutamyltranspeptidase, leucine aminopeptidase, acid phosphatase, cytokeratin, alpha 3,beta 1 integrin, and fibronectin.

The cells are negative for factor VIII related antigen, 6.19 antigen and CALLA endopeptidase.

HK-2 cells retain functional characteristics of proximal tubular epithelium such as Na+ dependent / phlorizin sensitive sugar transport and adenylate cyclase responsiveness to parathyroid, but not to antidiuretic hormone.

The cells are capable of gluconeogenesis as evidenced by their ability to make and store glycogen.

HK-2 cells are anchorage dependent.

The cells will not grow in methylcellulose, soft agar or suspension.

HK-2 cells can reproduce experimental results obtained with freshly isolated PTCs.



Propagation:ATCC complete growth medium: The base medium for this cell line is provided by Invitrogen (GIBCO) as part of a kit: Keratinocyte Serum Free Medium (K-SFM), Kit Catalog Number 17005-042. This kit is supplied with each of the two additives required to grow this cell line (bovine pituitary extract (BPE) and human recombinant epidermal growth factor (EGF). To make the complete growth medium, you will need to add the following components to the base medium:
  • 0.05 mg/ml BPE - provided with the K-SFM kit

  • 5 ng/ml EGF - provided with the K-SFM kit. NOTE: Do not filter complete medium.
    Atmosphere: air, 95%; carbon dioxide (CO2), 5%
    Temperature: 37.0°C
    Growth Conditions: Cell growth is dependent on epidermal growth factor. The cells should not be allowed to become confluent. Subculture at 80% of confluence.



Subculturing:Protocol:
  1. Remove and discard culture medium.

  2. Briefly rinse the cell layer with 0.05% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

  5. To remove trypsin-EDTA solution, transfer cell suspension to centrifuge tube and spin at approximay 125 xg for 5 to 10 minutes.Discard supernatant and resuspend cells in fresh growth medium. Add appropriate aliquots of cell suspension to new culture vessels.

  6. Incubate cultures at 37°C.






















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說明:

  • 驗(yàn)證碼:

    請輸入計(jì)算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號:滬ICP備10013034號-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
久久国产精品无码网站| 国产精品毛片一区二区| 男女高潮喷水在线观看| 国产精品一区二区亚洲| 亚洲乱码中文字幕久久孕妇黑人| 国产精品麻花传媒二三区别| 国产精品厕所| 白晶晶果冻传媒国产今日推荐| 中文字幕在线日亚洲9| 青青久在线视频免费观看| 国产精品内射久久久久欢欢| 99精品偷自拍| 猫咪www免费人成人入口| 中文字幕无码家庭乱欲| 国产高清精品房事自拍| 色综合色综合久久综合频道88| 久久人妻少妇嫩草av蜜桃| 小说区 综合区 首页| 国产香蕉一区二区三区在线视频| 一区二区三区婷婷在线| 国产未成女一区二区| 国产精品大片中文字幕| 我要看a级毛片| 亚洲精品国际精品乱码| 一边吃奶一边摸做爽视频| av中文字幕国产精品| 国产美女口爆吞精普通话| 国产在线精品综合色区| 国色天香精品一卡2卡3卡4| 黄片av毛片在线观看| 无码专区—va亚洲v天堂麻豆| 三级国产在线三级视频| 国产日韩精品一区二区三区在线 | 久久久久99精品国产片| 性视频播放免费视频| 伊人久久大香线蕉亚洲五月天| 国产97色在线 | 日韩| 亚洲国产精品成人久久久| 精精国产xxxx视频在线播放| 久久精品国产亚洲av电影网| 日日噜噜夜夜狠狠久久丁香五月|