国产亚洲精品福利视频在线观看|无遮挡边摸边吃奶边做视频免费|在办公室拨开老师内裤进入|国产日韩欧美一区二区东京热

咨詢熱線

15000266580

當(dāng)前位置:首頁(yè)  >  技術(shù)文章  >  4T1 小鼠乳腺癌細(xì)胞

4T1 小鼠乳腺癌細(xì)胞

更新時(shí)間:2016-06-22      點(diǎn)擊次數(shù):2468

細(xì)胞名稱:4T1 小鼠乳腺癌細(xì)胞
介紹:4T1 是從410.4瘤株中未經(jīng)誘變篩得的6-硫鳥嘌噙抗性細(xì)胞株。 當(dāng)注射到BALB/c 小鼠中時(shí),4T1自發(fā)產(chǎn)生高轉(zhuǎn)移腫瘤,可轉(zhuǎn)移到肺,肝,淋巴結(jié)和大腦,同時(shí)在注射部位形成始發(fā)灶。 誘導(dǎo)轉(zhuǎn)移時(shí)不需要摘除始發(fā)灶。 4T1細(xì)胞在BALB/c小鼠中的生長(zhǎng)與轉(zhuǎn)移特性與人體中的乳腺癌十分相近。這種腫瘤是人VI期乳腺癌的動(dòng)物模型。 4T1-誘導(dǎo)的腫瘤在手術(shù)后及未手術(shù)情況下轉(zhuǎn)移的動(dòng)力學(xué)相近,可以用作手術(shù)后及未手術(shù)模型。 跟其他腫瘤模型相比,由于4T1的抗6-硫鳥嘌噙特性,微小的轉(zhuǎn)移細(xì)胞團(tuán)(少到僅僅1個(gè))也可以在許多遠(yuǎn)端器官中檢測(cè)到。 沒必要數(shù)淋巴結(jié)或稱重器官
形態(tài)特性       上皮細(xì)胞
生長(zhǎng)特性      貼壁生長(zhǎng)
傳代方法       消化3-5分鐘。1:2。3天內(nèi)可長(zhǎng)滿。
支原體檢測(cè)      陰性
下面是ATCC的介紹

4T1 (ATCC® CRL-2539™)
Organism    Mus musculus, mouse
Tissue     mammary gland
Product Format     frozen4T1 小鼠乳腺癌細(xì)胞
Morphology     epithelial
Culture Properties     adherent
Biosafety Level     1
Disease     This tumor is an animal stage IV human breast cancer.
Strain     BALB/cfC3H
Applications    
The tumor growth and metastatic spread of 4T1 cells in BALB/c mice very closely mimic human breast cancer. This tumor is an animal model for stage IV human breast cancer.

4T1-induced tumors can be used as a post-operative model as well as a non-surgical model because the 4T1-induced tumor metastasizes spontaneously in both models with similar kinetics.
Storage Conditions     liquid nitrogen vapor temperature
Derivation    
4T1 is a 6-thioguanine resistant cell line selected from the 410.4 tumor without mutagen treatment.
Tumorigenic     Yes
Effects    4T1 小鼠乳腺癌細(xì)胞
Yes, forms tumors and metastasizes in BALB/c mice
Comments    
When injected into BALB/c mice, 4T1 spontaneously produces highly metastatic tumors that can metastasize to the lung, liver, lymph nodes and brain while the primary tumor is growing in situ.
The primary tumor does not have to be removed to induce metastatic growth.
Because 4T1 is resistant to 6-thioquanine, micro-metastatic cells (as few as 1) can be detected in many distant site organs with better accuracy that most tumor models. There is no need to count nodules or weight target organs.

Complete Growth Medium     The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Phenol Red is a structural mimic for estrogen. Therefore the estrogenic activity of phenol red should be considered in any studies that utilize estrogen-responsive cells in culture (Ref). The Phenol Red-free version of RPMI 1640, is ATCC catalog No. 30-2602.

Subculturing    
NOTE: the cells should not be allowed to become confluent, subculture at 80% of confluence. Remove medium, and rinse with 0.25% trypsin-0.53mM EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subc*tion Ratio: A subc*tion ratio of 1:6 to 1:8 is recommended
Medium Renewal: Every 2 to 3 days
Cryopreservation    
Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor temperature
Culture Conditions    
Temperature: 37°C

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
92国产精品午夜福利免费| 久久久久成人精品免费播放动漫| 国产果冻豆传媒麻婆精东| 欧美另类高清zo欧美| 国产麻豆黄色一级视频| 浪潮av激情高潮国产精品 | 国産精品久久久久久久| 精品人妻无码一区二区三区性| 国产不卡免费一区二区| 欧美性xxxxx极品娇小| 免费少妇荡乳情欲视频| 成人午夜又粗又硬又长| 国产精品久久国产精品99 gif| 亚洲一码二码在线观看| 日韩精品无码一本二本三本 | 亚洲日韩av无码中文字幕美国| 亚洲第一极品精品无码久久| 亚洲日韩欧美一区二区三区| 成人网站在线进入爽爽爽| 蜜桃视频在线观看免费视频网站www| 麻豆aⅴ精品无码一区二区| 无码人妻精品一二三区免费| 天堂一区二区三区av| 国产精品白丝av嫩草影院| 无码专区一va亚洲v专区在线| 国产精品亚洲片夜色在线| 亚洲av片无码久久五月| 欧美超大胆裸体xx视频| 性动态图av无码专区| 精品人妻伦九区久久aaa片69| 性色欲情网站| 日本aaaa片毛片免费观色戒| 女厕厕露p撒尿八个少妇| 人禽交 欧美 网站| 久久精品国产亚洲av影院| 饥渴少妇高潮视频大全| 久久亚洲精品无码播放| 久久亚洲精品ab无码播放| 黄色三级国产视频无毒| 开心久久婷婷综合中文字幕| 特黄做受又硬又粗又大视频小说|