国产亚洲精品福利视频在线观看|无遮挡边摸边吃奶边做视频免费|在办公室拨开老师内裤进入|国产日韩欧美一区二区东京热

咨詢熱線

15000266580

當前位置:首頁  >  技術文章  >  H9c2(2-1)大鼠心肌細胞的介紹

H9c2(2-1)大鼠心肌細胞的介紹

更新時間:2014-05-28      點擊次數(shù):3523

該細胞由Kimes B和Brandt B從BD1X大鼠胚胎心臟組織的克隆細胞株亞克隆得到;表現(xiàn)出許多骨骼肌的特性。這個細胞株中的成肌細胞能融合形成多核的肌管,并對乙酰膽堿的刺激發(fā)生反應。如果培養(yǎng)基中的血清濃度下降到1%,融合很快發(fā)生。
動物種別:大鼠。組織來源:心臟,心肌層。形態(tài):成肌細胞
DMEM高糖培養(yǎng)基(GIBCO,貨號11995065)+10%進口胎牛血清。
凍存條件:培養(yǎng)液+10%DMSO

H9c2(2-1)大鼠心肌細胞的介紹
以下是此細胞ATCC介紹
 

CRL-1446 H9c2(2-1) 大鼠心肌細胞 的詳細介紹
H9c2(2-1) 大鼠心肌細胞

ATCC® Number:  CRL-1446™       
Designations:  H9c2(2-1)  
Depositors:   W Carlisle  
Biosafety Level: 1  
Shipped:  frozen  
Medium & Serum:  See Propagation  
Growth Properties: adherent 
Organism: Rattus norvegicus (rat)  
Morphology: myoblast

 
Source: Strain: BD1X 
Organ: heart 
Tissue: myocardium 
Cellular Products: myokinase; creatine phosphokinase; myosin  
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.  
 
Applications: transfection host (Roche FuGENE® Transfection Reagents) 
Receptors: acetylcholine, expressed 
Age:  embryo  
Comments: H9c2(2-1) is a subclone of the original clonal cell line derived from embryonic BD1X rat heart tissue by B. Kimes and B. Brandt and exhibits many of the properties of skeletal muscle. 
Myoblastic cells in this line will fuse to form multinucleated myotubes and respond to acetylcholine stimulation. 
Fusion occurs faster if the serum concentration in the medium is reduced to one percent. 
Propagation:  ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5% 
Temperature: 37.0°C ?H9c2(2-1) 大鼠心肌細胞
Subculturing:  Protocol: The myoblastic population will become depleted rapidly if the cultures are allowed to become confluent.
To prevent loss of myoblastic cells, cultures should be subcultured before they become confluent, and the line should be recloned periodically with selection for myoblastic cells.

Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.

Subc*tion Ratio: A subc*tion ratio of 1:2 to 1:4 is recommended 
Medium Renewal: Every 2 to 3 days 
Preservation:  Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO 
Storage temperature: liquid nitrogen vapor phase 
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020   ?H9c2(2-1) 大鼠心肌細胞
References: 1062: Kimes BW, Brandt BL. Properties of a clonal muscle cell line from rat heart. Exp. Cell Res. 98: 367-381, 1976. PubMed: 943302
32970: Levy AP, et al. Post-transcriptional regulation of vascular endothelial growth factor by hypoxia. J. Biol. Chem. 271: 2746-2753, 1996. PubMed: 8576250 


 

聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復祥生物科技有限公司 All Right Reserved    備案號:滬ICP備10013034號-2     sitemap.xml
技術支持:化工儀器網(wǎng)    管理登陸
无码骚夜夜精品| 欧美又黄又大又爽a片三年片| 8ⅹ8x擦拨擦拨成人免费视频| 久久男人av资源网站无码软件| 国模叶桐尿喷337p人体| 5858s亚洲色大成网站www| 免费人成在线观看| 国产69精品久久久久人妻刘玥| 少妇装睡让我滑了进去| 人摸人人人澡人人超碰| 久久国产精品二国产精品| 欧美日韩精品一区二区视频| 成人性色生活片免费毛片| 国产精品亚洲а∨无码播放不卡| 中文无码一区二区不卡av| 国产成人亚洲精品青草天美| 日本不卡三区| 中文字幕久精品免费视频| 美女毛片一区二区三区四区| 国产成人精品视频ⅴa片软件竹菊 国产成人精品日本亚洲专区61 | 亚洲午夜精品久久久久久浪潮| 国产精品视频一区二区三区无码| 香蕉久久夜色精品国产| 色欲色香天天天综合vvv| 成人欧美一区二区三区| 麻豆妓女爽爽一区二区三| 国产麻豆出品在线观看av| 欧美牲交40_50a欧美牲交aⅴ| 国产一精品一av一免费爽爽| 国产精品无码mv在线观看| 伊人久久大香线蕉av仙人| 交换交换乱杂烩系列yy| 狂猛欧美激情性xxxx大豆行情| 亚洲夜夜性无码| 国产嫩草视频一区二区| 天堂www中文在线资源| 国产精品爆乳在线播放第一人称| 大陆极品少妇内射aaaaa| 麻豆人人妻人人妻人人片av| 国产一区二区三区在线观看免费 | 波多野成人无码精品电影|